Overexpression, Purification, and Characterization of Bacillus subtilis N-Acetylmuramoyl-<small>L</small>-alanine Amidase CwlC

Toshio SHIDA; Hiromi HATTORI; Fuminori ISE; Junichi SEKIGUCHI

doi:10.1271/bbb.64.1522

Biochemistry & Molecular Biology Notes

Overexpression, Purification, and Characterization of Bacillus subtilis N-Acetylmuramoyl-L-alanine Amidase CwlC

Toshio SHIDA, Hiromi HATTORI, Fuminori ISE, Junichi SEKIGUCHI

著者情報

キーワード: Bacillus subtilis, N-acetylmuramoyl-L-alanine amidase, CwlC, substrate specificity, circular dichroism

ジャーナルフリー

2000 年 64 巻 7 号 p. 1522-1525

DOI https://doi.org/10.1271/bbb.64.1522

詳細

抄録

N-acetylmuramoyl-L-alanine amidase CwlC of Bacillus subtilis was overproduced in Escherichia coli and purified 21-fold. The amidase hydrolyzed type A cell walls such as B. subtilis. The amidase bound slightly to the Microbacterium lacticum cell wall (type B), but did not entirely hydrolyze it. The presence of calcium or magnesium ion increased the resistance of the amidase to heat denaturation.

責任著者(Corresponding author)

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