Microbial Production of 2-Deoxyribose 5-Phosphate from Acetaldehyde and Triosephosphate for the Synthesis of 2′-Deoxyribonucleosides

Jun OGAWA; Kyota SAITO; Takafumi SAKAI; Nobuyuki HORINOUCHI; Takako KAWANO; Seiichiro MATSUMOTO; Mie SASAKI; Yoichi MIKAMI; Sakayu SHIMIZU

doi:10.1271/bbb.67.933

Microbiology & Fermentation Technology Preliminary Communication

Microbial Production of 2-Deoxyribose 5-Phosphate from Acetaldehyde and Triosephosphate for the Synthesis of 2′-Deoxyribonucleosides

Jun OGAWA, Kyota SAITO, Takafumi SAKAI, Nobuyuki HORINOUCHI, Takako KAWANO, Seiichiro MATSUMOTO, Mie SASAKI, Yoichi MIKAMI, Sakayu SHIMIZU

著者情報

キーワード: 2-deoxyriobse 5-phosphate, 2′-deoxyribonucleoside, deoxyriboaldolase, triosephosphate, Klebsiella pneumoniae

ジャーナルフリー

2003 年 67 巻 4 号 p. 933-936

DOI https://doi.org/10.1271/bbb.67.933

詳細

抄録

2-Deoxyribose 5-phosphate was produced from acetaldehyde and dihydroxyacetone phosphate via D-glyceraldehyde 3-phosphate by Klebsiella pneumoniae B-4-4 through deoxyriboaldolase- and triosephosphate isomerase-catalyzing reactions. Under the optimum conditions, 98.7 mM 2-deoxyribose 5-phosphate was produced from 200 mM acetaldehyde and 117 mM dihydroxyacetone phosphate in 2 h with a molar yield of 84%. The 2-deoxyriobse 5-phosphate produced was directly transformed to 2′-deoxyribonucleoside by phosphopentomutase- and nucleoside phosphorylase-catalyzing reactions.

責任著者(Corresponding author)

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