We estimated the subsite structure of the maltogenic amylase from Thermomonospora viridis TF-35 (TVA). TVA has six subsites, and the catalytic site is between the 3rd and 4th subsite. The subsite affinities, A-3, A-2, (A-1+A+1), A+2 and A+3 were calculated to be 0.47, 2.31, 0.49, 2.45, and 0.17 kcal mol-1, respectively.
