Protein–DNA Interactions under High-Pressure Conditions, Studied by Capillary Narrow-Tube Electrophoresis

Hiroaki KAWANO; Kaoru NAKASONE; Fumiyoshi ABE; Chiaki KATO; Yasuhiko YOSHIDA; Ron USAMI; Koki HORIKOSHI

doi:10.1271/bbb.69.1415

Biochemistry & Molecular Biology Notes

Protein–DNA Interactions under High-Pressure Conditions, Studied by Capillary Narrow-Tube Electrophoresis

Hiroaki KAWANO, Kaoru NAKASONE, Fumiyoshi ABE, Chiaki KATO, Yasuhiko YOSHIDA, Ron USAMI, Koki HORIKOSHI

著者情報

キーワード: high-pressure electrophoresis, high-pressure electrophoretic mobility shift assay, Shewanella violacea, NtrC protein

ジャーナルフリー

2005 年 69 巻 7 号 p. 1415-1417

DOI https://doi.org/10.1271/bbb.69.1415

詳細

抄録

The method of electrophoretic mobility shift assay under high-pressure conditions was improved using a high-pressure electrophoresis apparatus with capillary narrow-tube gel. It was found that the protein–DNA complex in the gel was stained as a high-resolution spot with ethidium bromide. Using this method, it was found that the behavior under high-pressure conditions of the protein–DNA complex composed of NtrC protein and its target promoter DNA is important for the pressure-regulated transcription process, and it was confirmed that the complex was dissociated above a pressure of 70 MPa.

責任著者(Corresponding author)

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