Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Regular Papers
Activation of Murine Peritoneal Macrophages by Water-Soluble Extracts of Bursaphelenchus xylophilus, a Pine Wood Nematode
Hiroaki KAJIAkihiro TAIKazufumi MATSUSHITAHiroshi KANZAKIItaru YAMAMOTO
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2006 Volume 70 Issue 1 Pages 203-210

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Abstract

In our previous study, water-soluble extracts from Bursaphelenchus xylophilus (B. xylophilus), a pine wood nematode, were shown to enhance interleukin (IL)-4 plus lipopolysaccharide-induced polyclonal immunoglobulin (Ig) E production in vitro in mice and to increase serum levels of an antigen-nonspecific IgE in vivo. Here we examined whether the nematode extracts stimulate immunofunctions of murine peritoneal macrophages. In both resident and inflammatory macrophages, Fcγ receptor-mediated phagocytosis was markedly activated by B. xylophilus extracts, while non-specific phagocytosis was not. The enhancement of specific phagocytosis was accompanied by an increase in the formation of IgG-Fcγ receptor rosettes. B. xylophilus extracts also stimulated IL-1β production in both types of macrophages, and enhanced NO production and mRNA expression of inflammatory cytokines in inflammatory macrophages. These results indicate that the extracts of B. xylophilus contain an activating substance(s) for immunofunctions in macrophages, besides an enhancing factor for polyclonal IgE production.

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© 2006 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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