Yeast Two-Hybrid Detection Systems That Are Highly Sensitive to a Certain Kind of Endocrine Disruptors

Haeng-Seog LEE; Shin-ichi SASAGAWA; Shigeaki KATO; Ryouichi FUKUDA; Hiroyuki HORIUCHI; Akinori OHTA

doi:10.1271/bbb.70.521

Microbiology & Fermentation Technology Notes

Yeast Two-Hybrid Detection Systems That Are Highly Sensitive to a Certain Kind of Endocrine Disruptors

Haeng-Seog LEE, Shin-ichi SASAGAWA, Shigeaki KATO, Ryouichi FUKUDA, Hiroyuki HORIUCHI, Akinori OHTA

著者情報

キーワード: endocrine disruptor, estrogen, yeast two-hybrid system, human estrogen receptor-α (hERα), steroid receptor co-activator-1 (SRC-1)

ジャーナルフリー

2006 年 70 巻 2 号 p. 521-524

DOI https://doi.org/10.1271/bbb.70.521

詳細

抄録

We tested the effects of several combinations of bait and fish components of the yeast two-hybrid detection system for estrogenic activity. A combination of the full-length human estrogen receptor α with the nuclear receptor-binding domain of co-activator steroid receptor co-activator-1 (SRC-1) or transcriptional intermediate factor-2 (TIF-2) was most effective for estrogen-dependent induction of the chromosome-integrated UAS_GAL-CYC1^p-lacZ reporter construct among the two-hybrid systems so far tested.

責任著者(Corresponding author)

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