High-Level Expression of Recombinant Active Human Renin in Sf-9 Cells: Rapid Purification and Characterization

Saori TAKAHASHI; Keishi HATA; Ken-Ichi KIKUCHI; Takeshi GOTOH

doi:10.1271/bbb.70416

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Biochemistry & Molecular Biology Notes

High-Level Expression of Recombinant Active Human Renin in Sf-9 Cells: Rapid Purification and Characterization

Saori TAKAHASHI, Keishi HATA, Ken-Ichi KIKUCHI, Takeshi GOTOH

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Keywords: renin, prorenin, expression, baculovirus, Sf-9 cells

JOURNAL FREE ACCESS

2007 Volume 71 Issue 10 Pages 2610-2613

DOI https://doi.org/10.1271/bbb.70416

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Abstract

Recombinant human (rh) renin was expressed in Sf-9 insect cells. Baculovirus-infected Sf-9 cells produced active rh-renin in the late stage of cultivation. The rh-renin was purified after 5 d of culture by two steps of column chromatography. Approximately 0.61 mg of pure rh-renin was obtained from 200 ml of culture medium with a yield of 35.3%.

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