A Multi-Fluorescent MDA435 Cell Line for Mitosis Inhibitor Studies: Simultaneous Visualization of Chromatin, Microtubules, and Nuclear Envelope in Living Cells

Takashi FUKADA; Kaori SENDA-MURATA; Kumi NISHIDA; Shinji SAKAUSHI; Harumi MINAMIKAWA; Masaya DOTSU; Shigenori OKA; Kenji SUGIMOTO

doi:10.1271/bbb.70355

Biochemistry & Molecular Biology Notes

A Multi-Fluorescent MDA435 Cell Line for Mitosis Inhibitor Studies: Simultaneous Visualization of Chromatin, Microtubules, and Nuclear Envelope in Living Cells

Takashi FUKADA, Kaori SENDA-MURATA, Kumi NISHIDA, Shinji SAKAUSHI, Harumi MINAMIKAWA, Masaya DOTSU, Shigenori OKA, Kenji SUGIMOTO

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Keywords: mitosis, microtubule, Taxol, fluorescent protein, time-lapse microscopy

JOURNAL FREE ACCESS

2007 Volume 71 Issue 10 Pages 2603-2605

DOI https://doi.org/10.1271/bbb.70355

View "Advance Publication" version (October 7, 2007).

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Abstract

As a part of our efforts to create a pre-made multi-fluorescent cell library for various cytological assays, we made a triple-fluorescent cell line in which microtubules, chromosomes, and nuclear envelopes were visualized for simultaneous observation of spindle structure and chromosome distribution in living cells. Pilot experiments with microtubule-disturbing drugs showed the advantages of this cell line in mitosis inhibitor studies.

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