Two Proteins with Diaphorase Activity from Clostridium thermocellum and Moorella thermoacetica

Saikat CHAKRABORTY; Makiko SAKKA; Tetsuya KIMURA; Kazuo SAKKA

doi:10.1271/bbb.70646

Microbiology & Fermentation Technology Note

Two Proteins with Diaphorase Activity from Clostridium thermocellum and Moorella thermoacetica

Saikat CHAKRABORTY, Makiko SAKKA, Tetsuya KIMURA, Kazuo SAKKA

著者情報

キーワード: diaphorase, Clostridium thermocellum, Moorella thermoacetica, rubredoxin, flavin reductase

ジャーナルフリー

2008 年 72 巻 3 号 p. 877-879

DOI https://doi.org/10.1271/bbb.70646

View "Advance Publication" version (March 7, 2008).

詳細

抄録

Two high-molecular-weight rubredoxin genes (hrb) from Clostridium thermocellum and Moorella thermoacetica were expressed in Escherichia coli and their translated products (Hrb) were characterized. M. thermoacetica Hrb showed strong diaphorase activity. In contrast, C. thermocellum Hrb, containing neither FMN nor FAD in the molecule, showed flavin reductase activity with moderate diaphorase activity. Both enzymes were optimally active at about 50 °C.

責任著者(Corresponding author)

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