Two Proteins with Diaphorase Activity from Clostridium thermocellum and Moorella thermoacetica

Saikat CHAKRABORTY; Makiko SAKKA; Tetsuya KIMURA; Kazuo SAKKA

doi:10.1271/bbb.70646

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Two Proteins with Diaphorase Activity from Clostridium thermocellum and Moorella thermoacetica

Saikat CHAKRABORTY, Makiko SAKKA, Tetsuya KIMURA, Kazuo SAKKA

著者情報

キーワード: diaphorase, Clostridium thermocellum, Moorella thermoacetica, rubredoxin, flavin reductase

ジャーナルフリー早期公開

論文ID: 70646

DOI https://doi.org/10.1271/bbb.70646

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2008) , No. 3 p.877

詳細

抄録

Two high-molecular-weight rubredoxin genes (hrb) from Clostridium thermocellum and Moorella thermoacetica were expressed in Escherichia coli and their translated products (Hrb) were characterized. M. thermoacetica Hrb showed strong diaphorase activity. In contrast, C. thermocellum Hrb, containing neither FMN nor FAD in the molecule, showed flavin reductase activity with moderate diaphorase activity. Both enzymes were optimally active at about 50 °C.

責任著者(Corresponding author)

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