Low-Molecular-Weight Hyaluronan Permeates through Human Intestinal Caco-2 Cell Monolayers via the Paracellular Pathway

Nozomi HISADA; Hideo SATSU; Akira MORI; Mamoru TOTSUKA; Jun-ichi KAMEI; Takashi NOZAWA; Makoto SHIMIZU

doi:10.1271/bbb.70748

Food & Nutrition Science Notes

Low-Molecular-Weight Hyaluronan Permeates through Human Intestinal Caco-2 Cell Monolayers via the Paracellular Pathway

Nozomi HISADA, Hideo SATSU, Akira MORI, Mamoru TOTSUKA, Jun-ichi KAMEI, Takashi NOZAWA, Makoto SHIMIZU

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Keywords: hyaluronan, Caco-2, tight junction, paracallular pathway

JOURNAL FREE ACCESS

2008 Volume 72 Issue 4 Pages 1111-1114

DOI https://doi.org/10.1271/bbb.70748

View "Advance Publication" version (April 7, 2008).

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Abstract

The intestinal permeability of low-molecular-weight hyaluronan (LMW-HA) was investigated by using cultured monolayers of Caco-2 cells. The amount of LMW-HA that permeated the Caco-2 monolayers was measured by a carbazole assay. The permeability of LMW-HA increased inversely with the molecular size and was dose-dependent. The transport was observed to be energy-independent, and was correlated with the tight junction (TJ) permeability. These results suggest that LMW-HA permeated the Caco-2 cell monolayers via the paracellular pathway.

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