Cloning, Expression and Purification of Bacillus cereus Endochitinase in the Escherichia coli AD494(DE3)pLysS Expression System

Wei-Ming CHEN; Gen-Hung CHEN; Ching-San CHEN; Shann-Tzong JIANG

doi:10.1271/bbb.80618

Biochemistry & Molecular Biology Notes

Cloning, Expression and Purification of Bacillus cereus Endochitinase in the Escherichia coli AD494(DE3)pLysS Expression System

Wei-Ming CHEN, Gen-Hung CHEN, Ching-San CHEN, Shann-Tzong JIANG

著者情報

キーワード: endochitinase, purification, recognition peptide, Bacillus cereus, Escherichia coli

ジャーナルフリー

2009 年 73 巻 5 号 p. 1172-1174

DOI https://doi.org/10.1271/bbb.80618

View "Advance Publication" version (May 7, 2009).

詳細

抄録

A chitinase gene from Bacillus cereus was cloned and expressed in Escherichia coli. The purified recombinant chitinase had much higher (128 fold) specificity to pNP-β-(GlcNAc)₃ than to pNP-β-(GlcNAc), suggesting endochitinase. Thirty-three amino acids in the N-terminal were recognized and cut off during expression, which consequently made the M_r not correspond to that predicted.

責任著者(Corresponding author)

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