Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Microbiology & Fermentation Technology Regular Papers
Identification of the Electron Transfer Flavoprotein as an Upregulated Enzyme in the Benzoate Utilization of Desulfotignum balticum
Hiroshi HABEAkinori KOBUNAAkifumi HOSODATomoyuki KOSAKATakayuki ENDOHHiroto TAMURAHisakazu YAMANEHideaki NOJIRIToshio OMORIKazuya WATANABE
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2009 Volume 73 Issue 7 Pages 1647-1652

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Abstract

Desulfotignum balticum utilizes benzoate coupled to sulfate reduction. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis was conducted to detect proteins that increased more after growth on benzoate than on butyrate. A comparison of proteins on 2D gels showed that at least six proteins were expressed. The N-terminal sequences of three proteins exhibited significant identities with the α and β subunits of electron transfer flavoprotein (ETF) from anaerobic aromatic-degraders. By sequence analysis of the fosmid clone insert (37,590 bp) containing the genes encoding the ETF subunits, we identified three genes, whose deduced amino acid sequences showed 58%, 74%, and 62% identity with those of Gmet_2267 (Fe-S oxidoreductase), Gmet_2266 (ETF β subunit), and Gmet_2265 (ETF α subunit) respectively, which exist within the 300-kb genomic island of aromatic-degradation genes from Geobacter metallireducens GS-15. The genes encoding ETF subunits found in this study were upregulated in benzoate utilization.

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© 2009 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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