Preparation and Epitope Mapping of a Monoclonal Antibody against Tri a Bd 27K, a Major Wheat Allergen

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We produced a monoclonal antibody (mAb) as a probe for detection of Tri a Bd 27K, a major wheat allergen. The mAb recognized the allergen purified from wheat flour, and the epitope on the allergen to the mAb was determined to be amino acid sequence ¹⁵⁴VPWVVVDGKPL¹⁶⁴ of Tri a Bd 27K. Of the amino acid residues on the epitope, the amino acid residues responsible for the binding to the mAb were found to be W156, D160, G161, and P163.