Preparation and Epitope Mapping of a Monoclonal Antibody against Tri a Bd 27K, a Major Wheat Allergen

Nobuko KOMIYAMA; Miki HIEMORI; Masumi KIMOTO; Makiko SUZUKI; Hiromi YAMASHITA; Yoshitaka TAKAHASHI; Hideaki TSUJI

doi:10.1271/bbb.90224

この記事には本公開記事があります。本公開記事を参照してください。
引用する場合も本公開記事を引用してください。

Preparation and Epitope Mapping of a Monoclonal Antibody against Tri a Bd 27K, a Major Wheat Allergen

Nobuko KOMIYAMA, Miki HIEMORI, Masumi KIMOTO, Makiko SUZUKI, Hiromi YAMASHITA, Yoshitaka TAKAHASHI, Hideaki TSUJI

著者情報

キーワード: wheat, allergen, Tri a Bd 27K, epitope structure, monoclonal antibody

ジャーナルフリー早期公開

論文ID: 90224

DOI https://doi.org/10.1271/bbb.90224

この記事には本公開記事があります。

The final version of this article is now available: Vol. 73 (2009) , No. 9 p.2113

詳細

抄録

We produced a monoclonal antibody (mAb) as a probe for detection of Tri a Bd 27K, a major wheat allergen. The mAb recognized the allergen purified from wheat flour, and the epitope on the allergen to the mAb was determined to be amino acid sequence ¹⁵⁴VPWVVVDGKPL¹⁶⁴ of Tri a Bd 27K. Of the amino acid residues on the epitope, the amino acid residues responsible for the binding to the mAb were found to be W156, D160, G161, and P163.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）