Preparation of Protein Transduction Domain-Fused Peptidyl Prolyl cis/trans Isomerase Pin1

Abstract

The phenotypes of mice lacking peptidyl prolyl cis/trans isomerase Pin1 (Pin1^−⁄−) indicated that deficient Pin1 might be related to a variety of diseases. We created TAT-Pin1, a fusion protein of human immunodeficiency virus 1 trans-activator of transcription factor with Pin1. Treatment of HeLa cells with TAT-Pin1 increased the ratio of the S phase. Moreover, TAT-Pin1 restored the proliferating function of Pin1^−⁄− mouse embryonic fibroblasts which cannot restart proliferation after G0 arrest. These results indicate that TAT-Pin1 is useful in studying the functions of Pin1 and can be developed as a macromolecular drug for diseases related to Pin1 loss.