Cloning of the Gene Encoding an Endo-Acting Pectate Lyase from Streptomyces thermocarboxydus

Akio TONOUCHI; Yuichi HARA; Ryousuke UMEHARA; Tamotsu SANUKI; Takuma FUKUSAWA; Kazuo MIYAIRI

doi:10.1271/bbb.90693

Biochemistry & Molecular Biology Notes

Cloning of the Gene Encoding an Endo-Acting Pectate Lyase from Streptomyces thermocarboxydus

Akio TONOUCHI, Yuichi HARA, Ryousuke UMEHARA, Tamotsu SANUKI, Takuma FUKUSAWA, Kazuo MIYAIRI

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Keywords: Streptomyces thermocarboxydus, pectate lyase, gene cloning, heterologous expression

JOURNAL FREE ACCESS

2010 Volume 74 Issue 2 Pages 433-436

DOI https://doi.org/10.1271/bbb.90693

View "Advance Publication" version (February 7, 2010).

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Abstract

An endo-acting family 3 pectate lyase (PL I) gene from Streptomyces thermocarboxydus was cloned and expressed in Escherichia coli. The deduced PL I sequence had highest similarity to a putative pectate lyase of S. coelicolor. Recombinant PL I had significantly lower specific activity, probably a result of incorrect folding, as indicated by circular dichroism spectra analysis.

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