A Simple in Situ Cell-Based SUMOylation Assay with Potential Application to Drug Screening

Miyuki MURAMATSU; Junsuke UWADA; Naoyuki MATSUMOTO; Hisato SAITOH

doi:10.1271/bbb.100081

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A Simple in Situ Cell-Based SUMOylation Assay with Potential Application to Drug Screening

Miyuki MURAMATSU, Junsuke UWADA, Naoyuki MATSUMOTO, Hisato SAITOH

著者情報

キーワード: post-translational modification, small ubiquitin-related modifier (SUMO), cell-based assay, drug-screening

ジャーナルフリー早期公開

論文ID: 100081

DOI https://doi.org/10.1271/bbb.100081

この記事には本公開記事があります。

The final version of this article is now available: Vol. 74 (2010) , No. 7 p.1473

詳細

抄録

We found that the small ubiquitin-related modifier (SUMO) conjugation reaction can be visualized simply by incubating green fluorescent protein (GFP)-SUMO-1 with permeabilized cells in the presence of ATP for 15 min. Neither special equipment for protein purification nor highly developed skills for recombinant technologies is required, making the assay potentially applicable to large-scale drug-screening strategies for the identification of drugs that can inhibit or enhance SUMOylation.

責任著者(Corresponding author)

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