A New Beneficial Mutation in Pseudomonas sp. 61-3 Polyhydroxyalkanoate (PHA) Synthase for Enhanced Cellular Content of 3-Hydroxybutyrate-Based PHA Explored Using Its Enzyme Homolog as a Mutation Template

Fumi SHOZUI; Jian SUN; Yuyang SONG; Miwa YAMADA; Kohei SAKAI; Ken’ichiro MATSUMOTO; Kazuma TAKASE; Seiichi TAGUCHI

doi:10.1271/bbb.100224

Microbiology & Fermentation Technology Notes

A New Beneficial Mutation in Pseudomonas sp. 61-3 Polyhydroxyalkanoate (PHA) Synthase for Enhanced Cellular Content of 3-Hydroxybutyrate-Based PHA Explored Using Its Enzyme Homolog as a Mutation Template

Fumi SHOZUI, Jian SUN, Yuyang SONG, Miwa YAMADA, Kohei SAKAI, Ken’ichiro MATSUMOTO, Kazuma TAKASE, Seiichi TAGUCHI

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Keywords: poly(3-hydroxybutyrate-co-3-hydroxyalkanoate), evolutionary engineering, PhaC1_Ps, 3-hydroxybutyrate-based copolymer

JOURNAL FREE ACCESS

2010 Volume 74 Issue 8 Pages 1710-1712

DOI https://doi.org/10.1271/bbb.100224

View "Advance Publication" version (August 7, 2010).

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Abstract

A newly isolated mutation (Gln508Leu) and a combination of it with previously discovered beneficial mutations in polyhydroxyalkanoate synthase 1 from Pseudomonas sp. 61-3 were found to enhance the production of poly(3-hydroxybutyrate) [P(3HB)] and poly(3HB-co-3-hydroxyalkanoate)s in recombinant Escherichia coli.

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