Production of P(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate) Terpolymers Using a Chimeric PHA Synthase in Recombinant Ralstonia eutropha and Pseudomonas putida

Jian SUN; Fumi SHOZUI; Miwa YAMADA; Ken’ichiro MATSUMOTO; Kazuma TAKASE; Seiichi TAGUCHI

doi:10.1271/bbb.100242

Microbiology & Fermentation Technology Notes

Production of P(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate) Terpolymers Using a Chimeric PHA Synthase in Recombinant Ralstonia eutropha and Pseudomonas putida

Jian SUN, Fumi SHOZUI, Miwa YAMADA, Ken’ichiro MATSUMOTO, Kazuma TAKASE, Seiichi TAGUCHI

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Keywords: chimeric enzyme, polyhydroxyalkanoate, PHA synthase, Pseudomonas putida, Aeromonas caviae

JOURNAL FREE ACCESS

2010 Volume 74 Issue 8 Pages 1716-1718

DOI https://doi.org/10.1271/bbb.100242

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Abstract

Recombinant strains of Ralstonia eutropha and Pseudomonas putida harboring a chimeric polyhydroxyalkanoate (PHA) synthase, which consisted of PHA synthases of Aeromonas caviae and R. eutropha, produced 3-hydroxybutyrate (3HB)-based PHA copolymers comprised of 3-hydroxyhexanoate and 3-hydroxyoctanoate units from dodecanoate (87–97 mol % 3HB), indicating that the chimeric PHA synthase possesses desirable substrate specificity leading to the production of 3HB-rich copolymers.

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