Molecular Cloning and Characterization of γ-Glutamyltranspeptidase from Pseudomonas nitroreducens IFO12694

Masashi IMAOKA; Shigekazu YANO; Masashi OKUMURA; Takao HIBI; Mamoru WAKAYAMA

doi:10.1271/bbb.100199

Biochemistry & Molecular Biology Notes

Molecular Cloning and Characterization of γ-Glutamyltranspeptidase from Pseudomonas nitroreducens IFO12694

Masashi IMAOKA, Shigekazu YANO, Masashi OKUMURA, Takao HIBI, Mamoru WAKAYAMA

著者情報

キーワード: Pseudomonas nitroreducens, γ-glutamyltranspeptidase, γ-glutamylmethylamide

ジャーナルフリー

2010 年 74 巻 9 号 p. 1936-1939

DOI https://doi.org/10.1271/bbb.100199

View "Advance Publication" version (September 7, 2010).

詳細

抄録

γ-Glutamyltranspeptidase from Pseudomonas nitroreducens IFO12694 (PnGGT) exhibited higher hydrolytic activity than transfer activity, as compared with other γ-glutamyltranspeptidases (GGTs). PnGGT showed little activity towards most of L-amino acids and towards glycyl-glycine, which is often used as a standard γ-glutamyl accepter in GGT transfer reactions. The preferred substrates for PnGGT as a γ-glutamyl accepter were amines such as methylamine, ethylamine, and isopropylamine.

責任著者(Corresponding author)

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