Atg22p, a Vacuolar Membrane Protein Involved in the Amino Acid Compartmentalization of Schizosaccharomyces pombe

Naoko SUGIMOTO; Tomoko IWAKI; Soracom CHARDWIRIYAPREECHA; Masamitsu SHIMAZU; Miyuki KAWANO; Takayuki SEKITO; Kaoru TAKEGAWA; Yoshimi KAKINUMA

doi:10.1271/bbb.100747

Microbiology & Fermentation Technology Notes

Atg22p, a Vacuolar Membrane Protein Involved in the Amino Acid Compartmentalization of Schizosaccharomyces pombe

Naoko SUGIMOTO, Tomoko IWAKI, Soracom CHARDWIRIYAPREECHA, Masamitsu SHIMAZU, Miyuki KAWANO, Takayuki SEKITO, Kaoru TAKEGAWA, Yoshimi KAKINUMA

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Keywords: Schizosaccharomyces pombe, vacuolar membrane, amino acid, transport

JOURNAL FREE ACCESS

2011 Volume 75 Issue 2 Pages 385-387

DOI https://doi.org/10.1271/bbb.100747

View "Advance Publication" version (February 7, 2011).

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Abstract

The fission yeast Schizosaccharomyces pombe has a homolog of the budding yeast Atg22p, which is involved in spore formation (Mukaiyama H. et al., Microbiology, 155, 3816–3826 (2009)). GFP-tagged Atg22p in the fission yeast was localized to the vacuolar membrane. Upon disruption of atg22, the amino acid levels of the cellular fraction as well as the vacuolar fraction decreased. The uptake of several amino acids, such as lysine, histidine, and arginine, was impaired in atg22Δ cells. S. pombe Atg22p plays an important role in the compartmentalization of amino acids.

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