A Calcium-Dependent Xylan-Binding Domain of Alkaline Xylanase from Alkaliphilic Bacillus sp. Strain 41M-1

Risa YAZAWA; Jun TAKAKURA; Tomoko SAKATA; IHSANAWATI; Rie YATSUNAMI; Toshiaki FUKUI; Takashi KUMASAKA; Nobuo TANAKA; Satoshi NAKAMURA

doi:10.1271/bbb.100730

Microbiology & Fermentation Technology Notes

A Calcium-Dependent Xylan-Binding Domain of Alkaline Xylanase from Alkaliphilic Bacillus sp. Strain 41M-1

Risa YAZAWA, Jun TAKAKURA, Tomoko SAKATA, IHSANAWATI, Rie YATSUNAMI, Toshiaki FUKUI, Takashi KUMASAKA, Nobuo TANAKA, Satoshi NAKAMURA

Author information

Risa YAZAWA
Department of Bioengineering, Tokyo Institute of Technology
Jun TAKAKURA
Department of Bioengineering, Tokyo Institute of Technology
Tomoko SAKATA
Department of Bioengineering, Tokyo Institute of Technology
IHSANAWATI
Department of Life Science, Tokyo Institute of Technology
Rie YATSUNAMI
Department of Bioengineering, Tokyo Institute of Technology
Toshiaki FUKUI
Department of Bioengineering, Tokyo Institute of Technology
Takashi KUMASAKA
Department of Life Science, Tokyo Institute of Technology
Nobuo TANAKA
Department of Life Science, Tokyo Institute of Technology
Satoshi NAKAMURA
Department of Bioengineering, Tokyo Institute of Technology

Keywords: xylanase, carbohydrate-binding module (CBM) family 36, xylan-binding domain, Bacillus sp.

JOURNAL FREE ACCESS

2011 Volume 75 Issue 2 Pages 379-381

DOI https://doi.org/10.1271/bbb.100730

View "Advance Publication" version (February 7, 2011).

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Abstract

Xylanase J of alkaliphilic Bacillus sp. strain 41M-1 contains a carbohydrate-binding module family 36 xylan-binding domain (XBD). Mutational analysis of the XBD revealed that Tyr237, Asp313, Trp317, and Asp318 were involved in Ca²⁺-dependent xylan-binding, and that Asp313 and Asp318 were especially important.

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