Pyrrolysine Analogs as Substrates for Bacterial Pyrrolysyl-tRNA Synthetase in Vitro and in Vivo

Hidekazu KATAYAMA; Kayo NOZAWA; Osamu NUREKI; Yoshiaki NAKAHARA; Hironobu HOJO

doi:10.1271/bbb.110653

Biochemistry & Molecular Biology Notes

Pyrrolysine Analogs as Substrates for Bacterial Pyrrolysyl-tRNA Synthetase in Vitro and in Vivo

Hidekazu KATAYAMA, Kayo NOZAWA, Osamu NUREKI, Yoshiaki NAKAHARA, Hironobu HOJO

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Keywords: Desulfitobacterium hafnience, pyrrolysine, pyrrolysyl-tRNA synthetase

JOURNAL FREE ACCESS

2012 Volume 76 Issue 1 Pages 205-208

DOI https://doi.org/10.1271/bbb.110653

View "Advance Publication" version (January 7, 2012).

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Abstract

Pyrrolysine-tRNA^Pyl complex is produced by pyrrolysyl-tRNA synthetase (PylRS). In this study, we investigated the substrate specificity of Desulfitobacterium hafnience PylRS. PylRS incorporated various L-lysine derivatives into tRNA^Pyl in vitro. In addition, the PylRS/tRNA^Pyl pair introduced these lysine derivatives into the recombinant protein by the Escherichia coli expression system, indicating that this PylRS/tRNA^Pyl pair can be used in protein engineering technology.

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