A Secreted Placental Alkaline Phosphatase-Based Reporter Assay System for Screening of Compounds Acting at an Octopamine Receptor Stably Expressed in a Mammalian Cell Line

Hiroto OHTA; Hiroshi OSHIUMI; Naotaka HAYASHI; Tetsuya IMAI; Yoshihisa OZOE; Shigeru MORIMURA; Kenji KIDA

doi:10.1271/bbb.110690

Biochemistry & Molecular Biology Notes

A Secreted Placental Alkaline Phosphatase-Based Reporter Assay System for Screening of Compounds Acting at an Octopamine Receptor Stably Expressed in a Mammalian Cell Line

Hiroto OHTA, Hiroshi OSHIUMI, Naotaka HAYASHI, Tetsuya IMAI, Yoshihisa OZOE, Shigeru MORIMURA, Kenji KIDA

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Keywords: insecticide, octopamine receptor, cAMP, secreted placental alkaline phosphatase (SEAP), reporter assay

JOURNAL FREE ACCESS

2012 Volume 76 Issue 1 Pages 209-211

DOI https://doi.org/10.1271/bbb.110690

View "Advance Publication" version (January 7, 2012).

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Abstract

Octopamine receptors are attractive insecticide targets. To screen compounds acting at octopamine receptors simply and rapidly, we constructed a chemiluminescent reporter gene assay system that detects secreted placental alkaline phosphatase transcriptionally regulated by the cAMP response element for a silkworm octopamine receptor. This system proved useful in high-throughput screening to develop octopamine receptor-specific insecticides.

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