Mevalonate-Dependent Enzymatic Synthesis of Amorphadiene Driven by an ATP-Regeneration System Using Polyphosphate Kinase

Manami SHIMANE; Yoshinori SUGAI; Ryosuke KAINUMA; Masahiro NATSUME; Hiroshi KAWAIDE

doi:10.1271/bbb.120177

Organic Chemistry Notes

Mevalonate-Dependent Enzymatic Synthesis of Amorphadiene Driven by an ATP-Regeneration System Using Polyphosphate Kinase

Manami SHIMANE, Yoshinori SUGAI, Ryosuke KAINUMA, Masahiro NATSUME, Hiroshi KAWAIDE

著者情報

キーワード: amorphadiene, ATP-regeneration, mevalonate pathway, polyphosphate kinase, ³¹P-NMR

ジャーナルフリー

2012 年 76 巻 8 号 p. 1558-1560

DOI https://doi.org/10.1271/bbb.120177

View "Advance Publication" version (August 7, 2012).

詳細

抄録

Polyphosphate kinase (PPK), which can regenerate ATP from ADP, was utilized in the mevalonate-dependent enzymatic synthesis of amorphadiene. The activity of PPK, cloned from Escherichia coli, was determined by ³¹P-NMR. The yield from the PPK-catalyzed synthesis was 25%, 2.5 times higher than that without PPK. The ³¹P-NMR analysis of the final reaction mixture indicated no accumulation of intermediates.

責任著者(Corresponding author)

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