The White-Rot Fungus Pleurotus ostreatus Transformant Overproduced Intracellular cAMP and Laccase

Yuki YAO; Takaiku SAKAMOTO; Yoichi HONDA; Yasuyuki KAGOTANI; Kousuke IZUMITSU; Kazumi SUZUKI; Toshikazu IRIE

doi:10.1271/bbb.130470

Microbiology & Fermentation Technology Notes

The White-Rot Fungus Pleurotus ostreatus Transformant Overproduced Intracellular cAMP and Laccase

Yuki YAO, Takaiku SAKAMOTO, Yoichi HONDA, Yasuyuki KAGOTANI, Kousuke IZUMITSU, Kazumi SUZUKI, Toshikazu IRIE

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Keywords: lignin, manganese peroxidase, laccase, mushroom

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Supplementary material

2013 Volume 77 Issue 11 Pages 2309-2311

DOI https://doi.org/10.1271/bbb.130470

View "Advance Publication" version (November 7, 2013).

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Abstract

Transformation of Pleurotus ostreatus PC9 with the mutated heterotrimeric G protein alpha subunit (Gα) gene resulted in higher laccase (Lac) activity and intracellular cyclic adenosine monophosphate (cAMP) concentrations as compared to those in wild-type PC9. The transformant also exhibited higher Lac activity than the wild type when cultured in a medium containing known Lac inducers CuSO₄ and ferulic acid.

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