Expression and Characterization of a Thermostable Acetylxylan Esterase from Caldanaerobacter subterraneus subsp. tengcongensis Involved in the Degradation of Insoluble Cellulose Acetate

Abstract

A thermostable acetylxylan esterase gene, TTE0866, which catalyzes the deacetylation of cellulose acetate, was cloned from the genome of Caldanaerobacter subterraneus subsp. tengcongensis. The pH and temperature optima were 8.0 and 60 °C. The esterase was inhibited by phenylmethylsulfonyl fluoride. A mixture of the esterase and cellulolytic enzymes efficiently degraded insoluble cellulose acetate with a higher degree of substitution.