Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
High-Yield Expression of Mouse Aos1-Uba2-Fusion SUMO-Activating Enzyme, mAU, in a Baculovirus-Insect Cell System
Ayumi KANEMARU, Hisato SAITOH
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キーワード: small ubiquitin-related modifier (SUMO), SUMO-activating enzyme (SUMO-E1), baculovirus-insect cell system, protein expression, post-translational protein modification
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2013 年 77 巻 7 号 p. 1575-1578

View "Advance Publication" version (July 7, 2013).
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Small ubiquitin-related modifier (SUMO) research requires large amounts of SUMO-activating enzyme (SUMO-E1) for in vitro SUMOylation assays. In this study the expression and purification of a hexahistidine-tagged mouse Aos1-Uba2-fusion protein (His6-mAU) in a baculovirus-insect cell system revealed that approximately 1.0 mg of highly-purified His6-mAU was obtained from 100 mL of Sf9 cell culture. The recombinant protein had SUMO-E1 activity in multiple in vitro SUMOylation assays.
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© 2013 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Agricultural and Biological Chemistry

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