High-Yield Expression of Mouse Aos1-Uba2-Fusion SUMO-Activating Enzyme, mAU, in a Baculovirus-Insect Cell System

Ayumi KANEMARU; Hisato SAITOH

doi:10.1271/bbb.130070

この記事には本公開記事があります。本公開記事を参照してください。
引用する場合も本公開記事を引用してください。

High-Yield Expression of Mouse Aos1-Uba2-Fusion SUMO-Activating Enzyme, mAU, in a Baculovirus-Insect Cell System

著者情報

キーワード: small ubiquitin-related modifier (SUMO), SUMO-activating enzyme (SUMO-E1), baculovirus-insect cell system, protein expression, post-translational protein modification

ジャーナルフリー早期公開

論文ID: 130070

DOI https://doi.org/10.1271/bbb.130070

この記事には本公開記事があります。

The final version of this article is now available: Vol. 77 (2013) , No. 7 p.1575

詳細

抄録

Small ubiquitin-related modifier (SUMO) research requires large amounts of SUMO-activating enzyme (SUMO-E1) for in vitro SUMOylation assays. In this study the expression and purification of a hexahistidine-tagged mouse Aos1-Uba2-fusion protein (His₆-mAU) in a baculovirus-insect cell system revealed that approximately 1.0 mg of highly-purified His₆-mAU was obtained from 100 mL of Sf9 cell culture. The recombinant protein had SUMO-E1 activity in multiple in vitro SUMOylation assays.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）