Effects of Modification of Amino Groups with Poly(Ethylene Glycol) on a Recombinant Uricase from Bacillus fastidiosus

Chun ZHANG; Xiaolan YANG; Juan FENG; Yonghua YUAN; Xiang LI; Youquan BU; Yanling XIE; Huidong YUAN; Fei LIAO

doi:10.1271/bbb.100080

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Effects of Modification of Amino Groups with Poly(Ethylene Glycol) on a Recombinant Uricase from Bacillus fastidiosus

Chun ZHANG, Xiaolan YANG, Juan FENG, Yonghua YUAN, Xiang LI, Youquan BU, Yanling XIE, Huidong YUAN, Fei LIAO

著者情報

キーワード: hyperuricemia, uricase, PEG modification, thermo-stability

ジャーナルフリー早期公開

論文ID: 100080

DOI https://doi.org/10.1271/bbb.100080

この記事には本公開記事があります。

The final version of this article is now available: Vol. 74 (2010) , No. 6 p.1298

詳細

抄録

After modification with monomethoxyl-poly(ethylene glycol)-5000, a recombinant intracellular uricase from Bacillus fastidiosus ATCC 29604 showed residual activity of about 65%, a thermo-inactivation half-life >85 h, a circulating half-life about 20 h in rats in vivo, consistent effects of common cations, and consistent optima for reaction temperature and pH. Thus, this uricase can be formulated via modification with monomethoxyl-poly(ethylene glycol).

責任著者(Corresponding author)

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