Characterization of an Aspergillus oryzae Cysteinyl Dipeptidase Expressed in Escherichia coli

Ryota HATTORI; Mayumi MATSUSHITA-MORITA; Junichiro MARUI; Sawaki TADA; Satoshi SUZUKI; Ikuyo FURUKAWA; Youhei YAMAGATA; Hitoshi AMANO; Hiroki ISHIDA; Michio TAKEUCHI; Ken-Ichi KUSUMOTO

doi:10.1271/bbb.100604

この記事には本公開記事があります。本公開記事を参照してください。
引用する場合も本公開記事を引用してください。

Characterization of an Aspergillus oryzae Cysteinyl Dipeptidase Expressed in Escherichia coli

Ryota HATTORI, Mayumi MATSUSHITA-MORITA, Junichiro MARUI, Sawaki TADA, Satoshi SUZUKI, Ikuyo FURUKAWA, Youhei YAMAGATA, Hitoshi AMANO, Hiroki ISHIDA, Michio TAKEUCHI, Ken-Ichi KUSUMOTO

著者情報

キーワード: Aspergillus oryzae, cysteinyl dipeptidase, metallopeptidase, characterization

ジャーナルフリー早期公開

論文ID: 100604

DOI https://doi.org/10.1271/bbb.100604

この記事には本公開記事があります。

The final version of this article is now available: Vol. 75 (2011) , No. 1 p.159

詳細

抄録

Cysteinyl dipeptidase from Aspergillus oryzae (CdpA) was produced in Escherichia coli and purified. The enzyme showed activity specific toward cysteine-containing dipeptides, but its substrate specificity was distinct from those of other cysteinyl dipeptidases of the M20 family. It was optimally active at pH 7–8 and stable at pH 6–9 and at up to 40 °C.

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