A Calcium-Dependent Xylan-Binding Domain of Alkaline Xylanase from Alkaliphilic Bacillus sp. Strain 41M-1

Risa YAZAWA; Jun TAKAKURA; Tomoko SAKATA; IHSANAWATI; Rie YATSUNAMI; Toshiaki FUKUI; Takashi KUMASAKA; Nobuo TANAKA; Satoshi NAKAMURA

doi:10.1271/bbb.100730

This article has now been updated. Please use the final version.

A Calcium-Dependent Xylan-Binding Domain of Alkaline Xylanase from Alkaliphilic Bacillus sp. Strain 41M-1

Risa YAZAWA, Jun TAKAKURA, Tomoko SAKATA, IHSANAWATI, Rie YATSUNAMI, Toshiaki FUKUI, Takashi KUMASAKA, Nobuo TANAKA, Satoshi NAKAMURA

Author information

Keywords: xylanase, carbohydrate-binding module (CBM) family 36, xylan-binding domain, Bacillus sp.

JOURNAL FREE ACCESS Advance online publication

Article ID: 100730

DOI https://doi.org/10.1271/bbb.100730

The final version of this article is now available: Vol. 75 (2011) , No. 2 p.379

Details

Abstract

Xylanase J of alkaliphilic Bacillus sp. strain 41M-1 contains a carbohydrate-binding module family 36 xylan-binding domain (XBD). Mutational analysis of the XBD revealed that Tyr237, Asp313, Trp317, and Asp318 were involved in Ca²⁺-dependent xylan-binding, and that Asp313 and Asp318 were especially important.

Corresponding author

Register with J-STAGE for free!