Mutants of Lactobacillus plantarum ML11-11 Deficient in Co-Aggregation with Yeast Exhibited Reduced Activities of Mixed-Species Biofilm Formation

Soichi FURUKAWA; Natsumi NOJIMA; Soma NOZAKA; Satoru HIRAYAMA; Ayumi SATOH; Hirokazu OGIHARA; Yasushi MORINAGA

doi:10.1271/bbb.110714

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Mutants of Lactobacillus plantarum ML11-11 Deficient in Co-Aggregation with Yeast Exhibited Reduced Activities of Mixed-Species Biofilm Formation

Soichi FURUKAWA, Natsumi NOJIMA, Soma NOZAKA, Satoru HIRAYAMA, Ayumi SATOH, Hirokazu OGIHARA, Yasushi MORINAGA

著者情報

キーワード: co-aggregation, mixed-species biofilm, Lactobacillus plantarum, Saccharomyces cerevisiae

ジャーナルフリー早期公開

論文ID: 110714

DOI https://doi.org/10.1271/bbb.110714

この記事には本公開記事があります。

The final version of this article is now available: Vol. 76 (2012) , No. 2 p.326

詳細

抄録

Lactic acid bacteria (LAB) mutants deficient in inter-species co-aggregation with yeast were spontaneously derived from Lactobacillus plantarum ML11-11, a significant mixed-species biofilm former in static co-cultures with budding yeasts. These non-co-aggregative mutants also showed significant decreases in mixed-species biofilm formation. These results suggest the important role of co-aggregation between LAB and yeast in mixed-species biofilm formation. Cell surface proteins obtained by 5 M LiCl extraction from the wild-type cells and non-co-aggregative mutant cells were analyzed by SDS–PAGE. There was an obvious difference in protein profiles. The protein band at 30 kDa was present abundantly in the wild-type cell surface fraction but was significantly decreased in the mutant cells. This band corresponded to the LAB surface factors that contribute to co-aggregation with yeasts.

責任著者(Corresponding author)

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