Development of an Enzyme-Linked Immunosorbent Assay System for the Determination of Asymmetric Dimethylarginine Using a Specific Monoclonal Antibody

Miyuki YOKORO; Makiko SUZUKI; Machiko YATANI; Hiromi YAMASHITA; Yoshitaka TAKAHASHI; Hideaki TSUJI; Masumi KIMOTO

doi:10.1271/bbb.110727

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Development of an Enzyme-Linked Immunosorbent Assay System for the Determination of Asymmetric Dimethylarginine Using a Specific Monoclonal Antibody

Miyuki YOKORO, Makiko SUZUKI, Machiko YATANI, Hiromi YAMASHITA, Yoshitaka TAKAHASHI, Hideaki TSUJI, Masumi KIMOTO

著者情報

キーワード: N^G,N^G-dimethyl-L-arginine, enzyme-linked immunosorbent assay, monoclonal antibody, endogenous inhibitor of NOS, risk factor for atherosclerosis

ジャーナルフリー早期公開

論文ID: 110727

DOI https://doi.org/10.1271/bbb.110727

この記事には本公開記事があります。

The final version of this article is now available: Vol. 76 (2012) , No. 2 p.400

詳細

抄録

We produced a monoclonal antibody (mAb) against N^G,N^G-dimethyl-L-arginine (asymmetric dimethylarginine: ADMA), an endogenous competitive inhibitor of nitric oxide synthase (NOS), and developed an enzyme-linked immunosorbent assay (ELISA). The competitive ELISA method using the mAb determined 5 nM–100 nM ADMA, and ADMA levels in human plasma and urine were found to be 0.78 μM and 51.3 μmol/g of creatinine respectively.

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