An Assay for Carbohydrate-Binding Activity of Lectins Using Polyamidoamine Dendrimer Conjugated with Carbohydrates

Tomomitsu HATAKEYAMA; Ryota KARINO; Yasuaki TERAI; Masanari KIMURA; Hideaki UNNO; Shuichiro GODA

doi:10.1271/bbb.120520

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An Assay for Carbohydrate-Binding Activity of Lectins Using Polyamidoamine Dendrimer Conjugated with Carbohydrates

Tomomitsu HATAKEYAMA, Ryota KARINO, Yasuaki TERAI, Masanari KIMURA, Hideaki UNNO, Shuichiro GODA

著者情報

キーワード: lectin, polyamidoamine dendrimer, carbohydrate, binding, light scattering

ジャーナルフリー早期公開

論文ID: 120520

DOI https://doi.org/10.1271/bbb.120520

この記事には本公開記事があります。

The final version of this article is now available: Vol. 76 (2012) , No. 10 p.1999

詳細

抄録

The carbohydrate-binding activity of lectins was examined using polyamidoamine dendrimer conjugated with carbohydrates (sugar-PD). When a C-type lectin, CEL-IV, was mixed with melibiose-PD, large complexes with a diameter of about 1 μm were formed. Changes in the amount of CEL-IV/melibiose-PD complex as an indication of lectin activity were measured sensitively by Rayleigh scattering. The carbohydrate specificity of the lectin was determined on the basis of inhibition of complex-formation by individual carbohydrates. It is suggested that various lectins can also be measured using sugar-PDs to which different carbohydrates are attached.

責任著者(Corresponding author)

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