Yeast Cell-Surface Expression of Chitosanase from Paenibacillus fukuinensis

Takeshi FUKUDA; Danya ISOGAWA; Madoka TAKAGI; Michiko KATO-MURAI; Hisashi KIMOTO; Hideo KUSAOKE; Mitsuyoshi UEDA; Shin-ichiro SUYE

doi:10.1271/bbb.70315

この記事には本公開記事があります。本公開記事を参照してください。
引用する場合も本公開記事を引用してください。

Yeast Cell-Surface Expression of Chitosanase from Paenibacillus fukuinensis

Takeshi FUKUDA, Danya ISOGAWA, Madoka TAKAGI, Michiko KATO-MURAI, Hisashi KIMOTO, Hideo KUSAOKE, Mitsuyoshi UEDA, Shin-ichiro SUYE

著者情報

キーワード: chitosanase, Paenibacillus fukuinensis, family 8 glycosyl hydrolase, yeast cell-surface engineering

ジャーナルフリー早期公開

論文ID: 70315

DOI https://doi.org/10.1271/bbb.70315

この記事には本公開記事があります。

The final version of this article is now available: Vol. 71 (2007) , No. 11 p.2845

詳細

抄録

To produce chitoorigosaccharides using chitosan, we attempted to construct Paenibacillus fukuinensis chitosanase-displaying yeast cells as a whole-cell biocatalyst through yeast cell-surface engineering. The localization of the chitosanase on the yeast cell surface was confirmed by immunofluorescence labeling of cells. The chitosanase activity of the constructed yeast was investigated by halo assay and the dinitrosalicylic acid method.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）