Purification and Characterization of a Rice Class I Chitinase, OsChia1b, Produced in Esherichia coli

Ryoji MIZUNO; Yoshikane ITOH; Yoko NISHIZAWA; Yuichiro KEZUKA; Kazushi SUZUKI; Takamasa NONAKA; Takeshi WATANABE

doi:10.1271/bbb.70693

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引用する場合も本公開記事を引用してください。

Purification and Characterization of a Rice Class I Chitinase, OsChia1b, Produced in Esherichia coli

Ryoji MIZUNO, Yoshikane ITOH, Yoko NISHIZAWA, Yuichiro KEZUKA, Kazushi SUZUKI, Takamasa NONAKA, Takeshi WATANABE

著者情報

キーワード: family 19 chitinase, antifungal activity, Oryza sativa

ジャーナルフリー早期公開

論文ID: 70693

DOI https://doi.org/10.1271/bbb.70693

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2008) , No. 3 p.893

詳細

抄録

To determine the properties and structure of OsChia1b, a family 19 chitinase from Oryza sativa L. cv. Nipponbare (japonica ssp.), recombinant OsChia1b was produced in Esherichia coli cells and purified to homogeneity by chitin affinity column chromatography. OsChia1b was highly active against soluble chitinous substrate, but not against crystalline chitin, and clearly inhibited hyphal extension of Trichoderma reesei.

責任著者(Corresponding author)

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