Functional Identification of a Rice ent-Kaurene Oxidase, OsKO2, Using the Pichia pastoris Expression System

Kwang-Wook KO; Fengqiu LIN; Takumi KATSUMATA; Yoshinori SUGAI; Sho MIYAZAKI; Hiroshi KAWAIDE; Kazunori OKADA; Hideaki NOJIRI; Hisakazu YAMANE

doi:10.1271/bbb.80427

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Functional Identification of a Rice ent-Kaurene Oxidase, OsKO2, Using the Pichia pastoris Expression System

Kwang-Wook KO, Fengqiu LIN, Takumi KATSUMATA, Yoshinori SUGAI, Sho MIYAZAKI, Hiroshi KAWAIDE, Kazunori OKADA, Hideaki NOJIRI, Hisakazu YAMANE

著者情報

キーワード: ent-kaurene oxidase, cytochrome P450 monooxygenase (P450), rice

ジャーナルフリー早期公開

論文ID: 80427

DOI https://doi.org/10.1271/bbb.80427

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2008) , No. 12 p.3285

詳細

抄録

Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis. We found that microsomes from the methylotropic yeast Pichia pastoris expressing both OsKO2 and a fungal cytochrome P450 monooxygenase (P450) reductase converted ent-kaurene to ent-kaurenoic acid. This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.

責任著者(Corresponding author)

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