Comparison of Luminescent Immunoassays Using Biotinylated Proteins of Aequorin, Alkaline Phosphatase and Horseradish Peroxidase as Reporters

Satoshi INOUYE; Jun-ichi SATO

doi:10.1271/bbb.80524

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Comparison of Luminescent Immunoassays Using Biotinylated Proteins of Aequorin, Alkaline Phosphatase and Horseradish Peroxidase as Reporters

Satoshi INOUYE, Jun-ichi SATO

著者情報

キーワード: streptavidin-biotin complex, chemical conjugation, aequorin, alkaline phosphatase, chemiluminescent immunoassay

ジャーナルフリー早期公開

論文ID: 80524

DOI https://doi.org/10.1271/bbb.80524

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2008) , No. 12 p.3310

詳細

抄録

We compare aequorin, alkaline phosphatase and horseradish peroxidase as reporters for luminescent immunoassays using α-fetoprotein (AFP) as a model analyte. Biotinylated aequorin was prepared from mutated aequorin containing a reactive cysteine residue by chemical conjugation. The measurable range of AFP using this biotinylated aequorin was 0.02–200 ng/ml, with a lower background level than the other biotinylated enzymes tested.

責任著者(Corresponding author)

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