Identification of a New Pheromone-Binding Protein in the Antennae of a Geometrid Species and Preparation of Its Antibody to Analyze the Antennal Proteins of Moths Secreting Type II Sex Pheromone Components

Hayaki WATANABE; Hiroko TABUNOKI; Nami MIURA; Aya MATSUI; Ryoichi SATO; Tetsu ANDO

doi:10.1271/bbb.90029

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Identification of a New Pheromone-Binding Protein in the Antennae of a Geometrid Species and Preparation of Its Antibody to Analyze the Antennal Proteins of Moths Secreting Type II Sex Pheromone Components

Hayaki WATANABE, Hiroko TABUNOKI, Nami MIURA, Aya MATSUI, Ryoichi SATO, Tetsu ANDO

著者情報

キーワード: pheromone perception, antennal protein, PCR, cDNA cloning, Geometridae in Lepidoptera

ジャーナルフリー早期公開

論文ID: 90029

DOI https://doi.org/10.1271/bbb.90029

この記事には本公開記事があります。

The final version of this article is now available: Vol. 73 (2009) , No. 6 p.1443

詳細

抄録

The full-length cDNA sequence of a new pheromone-binding protein (AscrPBP2) was determined from a geometrid moth, Ascotis selenaria cretacea, which secreted a Type II sex pheromone, and an antiserum against its recombinant protein overexpressed in Escherichia coli was prepared. In addition to this antiserum against AscrPBP2, antibodies against AscrPBP1 and general odorant-binding proteins of Bombyx mori were used in Western blotting experiments to analyze the proteins in the antennae of several lepidopteran species secreting Type II sex pheromone components.

責任著者(Corresponding author)

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