Piezo-Adapted 3-Isopropylmalate Dehydrogenase of the Obligate Piezophile Shewanella benthica DB21MT-2 Isolated from the 11,000-m Depth of the Mariana Trench

Ryota KASAHARA; Takako SATO; Hideyuki TAMEGAI; Chiaki KATO

doi:10.1271/bbb.90448

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Piezo-Adapted 3-Isopropylmalate Dehydrogenase of the Obligate Piezophile Shewanella benthica DB21MT-2 Isolated from the 11,000-m Depth of the Mariana Trench

Ryota KASAHARA, Takako SATO, Hideyuki TAMEGAI, Chiaki KATO

著者情報

キーワード: 3-isopropylmalate dehydrogenase, obligate piezophile, adaptation to high pressure

ジャーナルフリー早期公開

論文ID: 90448

DOI https://doi.org/10.1271/bbb.90448

この記事には本公開記事があります。

The final version of this article is now available: Vol. 73 (2009) , No. 11 p.2541

詳細

抄録

3-Isopropylmalate dehydrogenase (IPMDH)-encoding leuB genes were obtained from the obligate piezophile Shewanella benthica DB21MT-2 and non-piezophile Shewanella oneidensis MR-1. The genes were expressed in Escherichia coli and the proteins were purified using His-tag. The estimated kinetic parameters of these enzymes indicated that IPMDH of S. benthica DB21MT-2 is more tolerant of high pressure than that of S. oneidensis MR-1. Thus such an adaptation is one of the mechanisms bacteria utilize for survival at high pressures.

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