Repeated Batch Production of Theanine by Coupled Fermentation with Energy Transfer Using Membrane-Enclosed γ-Glutamylmethylamide Synthetase and Dried Yeast Cells

Sachiko YAMAMOTO; Yosuke MORIHARA; Mamoru WAKAYAMA; Takashi TACHIKI

doi:10.1271/bbb.90647

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Repeated Batch Production of Theanine by Coupled Fermentation with Energy Transfer Using Membrane-Enclosed γ-Glutamylmethylamide Synthetase and Dried Yeast Cells

Sachiko YAMAMOTO, Yosuke MORIHARA, Mamoru WAKAYAMA, Takashi TACHIKI

著者情報

キーワード: theanine, γ-glutamylmethylamide synthetase, coupled fermentation with energy transfer, repeated batch reaction

ジャーナルフリー早期公開

論文ID: 90647

DOI https://doi.org/10.1271/bbb.90647

この記事には本公開記事があります。

The final version of this article is now available: Vol. 73 (2009) , No. 12 p.2800

詳細

抄録

γ-Glutamylmethylamide synthetase and dried baker’s yeast cells were enclosed together in a dialysis membrane tube to produce theanine repeatedly by coupled fermentation with energy transfer. The membrane-enclosed enzyme preparation (M-EEP) formed approximately 600 mM theanine from glutamic acid and ethylamine at a 100% conversion rate. M-EEP maintained its productivity of theanine during six consecutive reactions in a mixture containing NAD⁺.

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