抄録
1. Polarography of each fraction isolated from sarkomycin by the countercurrent distri-bution method revealed the existence of two components, showing polarographic waves. One of them shows biological activity, while the other shows no biological activity.
2. The pure sarkomycin obtained by the countercurrent distribution method, showed two reduction waves in the acid region and one wave in neutral and alkaline regions, resulting in the approach of both waves.
3. Analysis of the slope of the reducing waves of sarkomycin gives values of 0.063 v. (for the first wave) and 0.076 v. (for the second).
4. Crystals Z and D, which are considered as degradation or polymerization products, were polarographed and showed one polaro-graphic wave in the acid region.
5. 3-Methyl-2-oxocyclopentane-l-carboxylic acid was polarographed and showed one wave below pH 4.0.
6. The correlation between reducing cur-rents and concentrations of sarkomycin solu-tion is linear, restrictive to the same sample. However, polarographic method can not be applied for determination, because the decrease rate of biological activity is not proportional to that of the reducing current.
