Specificity of a Tween-Lipase in Hydrolysis of Olive Oil

Abstract

A specific lipase for the hydrolysis of Tween present in a Sclerotinia lipase preparation was fractionated by various extraction procedures, ion-change resin treatment and dialysis. The activity of the Tween-lipase on olive oil was significant in increasing the hydrolysis of the olive oil by combination with various other lipases. Also, it seemed to develop with the change in the state of emulsion of the reaction mixture during hydrolysis.