Abstract
Many workers found that the poisoning animals with sodium fluoroacetate led quickly toa marked increase of citrate in the various tissues, but it failed to find such result in the liverin vivo as well as in vitro. In the liver of a frog, however, an accumulation of a large amountof citric acid was found 6 hours after the poison was orally administered to the animal. Todecide whether the accumulation of citric acid in the liver of a frog is due to the formation offluorocitrate in the liver itself or to the transport of fluorocitrate formed in other organs intothe liver, we carried out the experiments using the mitochondrial particles prepared from afrog liver. And it was found that the oxygen uptake of mitochondria was inhibited and thatthe citrate synthesis of the particle increased by the addition of fluoroacetate with pyruvateand malate as substrates.
