Enzymatic Conversion of D-Glucose to D-Fructose

Part V Partial Purification and Properties of the Enzyme from Aerobacter cloacae

抄録

Glucose isomerizing enzyme was partially purified after investigation on the properties of crude enzyme extract. The crude extract was partly inactivated by the contact with air. The addition of manganese was effective to improve the stability. Magnesium was essential to the enzyme action and cobalt accelerated the reaction.
The maximal activity was observed at pH about 7.6 and 50°C was optimal for the incubation time of 30 minutes. The enzyme solution reacted with D-xylose as well as D-glucose. The activity of the enzyme was inhibited at high glucose concentrations.