Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Studies on Metabolic Pathway of NAD in Yeast Part I
Partial Separation of Enzymes
Shuzo TAKEIJunichi TOTSUKazuo NAKANISHI
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1966 年 30 巻 2 号 p. 169-175

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The enzyme system of NAD degradation was extracted from autolysate of Saccharomyces oviformis.
The enzymes were partially separated by ammonium sulfate fractionation and DEAE-cellulose column chromatography, and then the metabolic pathway of NAD in yeast was presented, in which four enzymes were contained. It has been found that, among them, the 5'nucleotidase has more affinity for AMP and the nucleosidase has strict affinity for nicotinamide riboside.
In the degradation of NAD in the yeast, nucleotide pyrophosphatase was main enzyme, but NADase, nucleotide pyrophosphorylase and adenosine deaminase seemed not to play an important role.
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