Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Studies on the Acid-protease of Paecilomyces varioti BAINIER TPR-220 Part IV
Physical and Chemical Properties of the Crystalline Acid-protease
Jiro SAWADA
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1966 年 30 巻 4 号 p. 393-398

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Of the crystalline acid-protease from Paecilo-myces varioti BAINIER TPR-220, the chemical and physical properties were studied.
1. The sedimentation coefficient was 2.75×10-3 (s15), molecular weight 37, 500, isoelectric point at pH 3.8, and maximal absorption of ultraviolet spectrum at 280mμ.
2. The enzyme protein was consisted of 340 residues of amino acids, including 1 resi-due of cysteine and no cystine. Total number of acidic amino acid residues is greater than that of basic ones.
3. Determinations of N- and C-terminal amino acids were carried out.
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