抄録
Experiments were carried out to elucidate how paracasein was hydrolyzed by the intra-cellular protease of lactic acid bacteria (IPLB) prepared from S. cremoris and other three kinds of lactic acid bacteria. Various para-caseins prepared by exposing casein for variouss time were used as substrates.
These results show that paracaseins prepared by releasing of non casein type nitrogen (NCN) of 3_??_7% from native casein were hydrolyzed by IPLB more hardly than those prepared by releasing of 7% NCN or above-This phenomenon was not found in the ex-periment which was carried out by a neutrar. protease.
Results of DEAE-cellulose column chroma-tography or agar-gel or starch-gel electrophore-sis showed that rennin or IPLB of S. cremoris hydrolyzed some parts of αs-casein during the early stage of hydrolysis. During that stage-β-casein was also hydrolyzed by IPLB of S. cremoris.
